Coding

Part:BBa_K4739005:Design

Designed by: Huaiyuan Ma   Group: iGEM23_SUSTech-Shenzhen   (2023-10-09)


VgrG3-Dulaglutide fusion protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2761
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2761
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2761
    Illegal NgoMIV site found at 2818
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2299
    Illegal SapI.rc site found at 2736


Design Notes

1. Choose codons that are frequently used in the host organism to ensure efficient translation. 2. Optimize for codons corresponding to abundant tRNAs in the host to enhance translation speed. 3. Limit repetitive DNA sequences that may lead to recombination events and genomic instability.


Source

Sequence of VgrG3 gene is from V. cholerae genome, sequence of Dulaglutide is built from its amino acid sequence.

References